Fish oil supplementation induces Expression of genes related to cell cycle, endoplasmic reticulum stress and apoptosis in peripheral blood mononuclear cells: a transcriptomic approach

摘要

Background. Fish oil supplementation has been shownto alter gene expression of mononuclear cells both invitro and in vivo. However, little is known about thetotal transcriptome profile in healthy subjects afterintake of fish oil. We therefore investigated the geneexpression profile in peripheral blood mononuclearcells (PBMCs) after intake of fish oil for 7 weeksusing transcriptome analyses.Design. In a 7-week, double-blinded, randomized, controlled,parallel-group study, healthy subjectsreceived 8 g day 1 fish oil (1.6 g day 1 eicosapentaenoicacid + docosahexaenoic acid) (n = 17) or8 g day 1 high oleic sunflower oil (n = 19). Microarrayanalyses of RNA isolated from PBMCs were performedat baseline and after 7 weeks of intervention. Results. Cell cycle, DNA packaging and chromosomeorganization are biological processes found to beupregulated after intake of fish oil compared tohigh oleic sunflower oil using a moderated t-test.In addition, gene set enrichment analysis identifiedseveral enriched gene sets after intake of fishoil. The genes contributing to the significantlydifferent gene sets in the subjects given fish oilcompared with the control group are involved incell cycle, endoplasmic reticulum (ER) stress andapoptosis. Gene transcripts with common motifsfor 35 known transcription factors including E2F,TP53 and ATF4 were upregulated after intake offish oil.Conclusion. We have shown that intake of fish oil for7 weeks modulates gene expression in PBMCs ofhealthy subjects. The increased expression ofgenes related to cell cycle, ER stress and apoptosissuggests that intake of fish oil may modulate basiccellular processes involved in normal cellular function.